Controlled Immobilization Strategies to Probe Short Hyaluronan-Protein Interactions

Controlled Immobilization Strategies to Probe Short Hyaluronan-Protein Interactions.

Well-controlled grafting of small hyaluronan oligosaccharides (sHA) enables novel approaches to investigate biological processes such as angiogenesis, immune reactions and cancer metastasis. We develop two strategies for covalent attachment of sHA, a fast high-density adsorption and a two-layer system that allows tuning the density and mode of immobilization. We monitored the sHA adlayer format...

Capacitance DNA bio-chips improved by new probe immobilization strategies

Label-free DNA detection plays a crucial role in developing point-of-care biochips. Capacitance detection is a promising technology for label-free detection. However, data published in literature often show evident time drift, large standard deviation, scattered data points, and poor reproducibility. To address these problems, mercapto-hexanol or similar alkanethiols are usually considered as b...

Green fluorescent protein chimeras to probe protein-protein interactions.

Linker immobilization of protein and oligonucleotide on indium-tin-oxide for detection of probe-target interactions by Kelvin physics.

This work describes a label-free microarray analysis technique capable of detecting biomolecule target interactions with probes being anchored with a new linker-diluent system on indium-tin-oxide. The method is based on the differential work function characteristics of the substrate measured by a scanning Kelvin nanoprobe in terms of contact potential difference signals.

Affinity Interactions as a Tool for Protein Immobilization

Proteins, biopolymers composed of proteinogenic amino acids, are molecules with irreplaceable functions in human organism. They have among others a role as structural components, they are involved in motion processes, they appear as significant parts of immune response and different types of protection events, they play important roles in transport and storage processes and they also occur in s...